TurboGFP


Green fluorescent protein TurboGFP

- Extra fast protein maturation
- Bright green fluorescence
- Proven suitability to generate stably transfected cell lines
- Efficient maturation at a wide range of temperatures

TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

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TurboGFP expression/source vectors

pTurboGFP-C FP511 Mammalian expression vector encoding humanazied TurboGFP and allowing its expression and generation of fusions to the TurboGFP C-terminus 20 μg € 400.00
pTurboGFP-N FP512 Mammalian expression vector encoding humanazied TurboGFP and allowing its expression and generation of fusions to the TurboGFP N-terminus 20 μg € 400.00
pTurboGFP-B FP513 Bacterial expression vector; source of the TurboGFP coding sequence 20 μg € 400.00
pTurboGFP-PRL FP515 Promoterless vector encoding humanazied TurboGFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations 20 μg € 400.00
pTurboGFP-mito FP517 Mammalian expression vector encoding humanazied TurboGFP targeted to mitochondria 20 μg € 400.00
pTurboGFP-PRL-dest1 FP518 Promoterless vector encoding destabilized TurboGFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations 20 μg € 400.00
pTurboGFP-dest1 FP519 Mammalian expression vector encoding destabilized TurboGFP for its expression and generation of fusions to the TurboGFP-dest1 N-terminus 20 μg € 400.00
Gateway® TurboGFP-C entry clone FP521 Gateway® entry clone for generation of fusions to the C-terminus of humanazied TurboGFP; transfer of TurboGFP or TurboGFP-tagged fusion into a Gateway® destination vector 20 μg € 400.00
Gateway® TurboGFP-N entry clone FP522 Gateway® entry clone for generation of fusions to the N-terminus of humanazied TurboGFP; transfer of TurboGFP or TurboGFP-tagged fusion into a Gateway® destination vector 20 μg € 400.00
p2FP-RNAi FP981 Mammalian expression vector for RNAi studies 20 μg € 400.00


Vector sets

Cell-Killer FPK01 pKillerRed-dMito vector encoding mitochondria-targeted KillerRed and pTurboGFP-N vector allowing cytoplasmic expression of bright green fluorescent protein TurboGFP for monitoring cell fate 20 μg each € 650.00
Membrane-Killer FPK02 pKillerRed-mem vector encoding membrane-targeted KillerRed and pTurboGFP-N vector allowing cytoplasmic expression of bright green fluorescent protein TurboGFP for monitoring cell fate 20 μg each € 650.00
Mito-tracker FPM01 Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito 20 μg each € 700.00
Promoter-tracker Green FPP03 Promoterless vectors pTurboGFP-PRL, pTurboGFP-PRL-dest1, and control vector pTurboGFP-dest1 20 μg each € 700.00
Promoter-tracker 3-colors FPP15 Promoterless vectors pTurboYFP-PRL, pTurboGFP-PRL, and pTurboRFP-PRL 20 μg each € 700.00


Recombinant protein

rTurboGFP FP552 Purified recombinant bright green fluorescent protein 100 μg € 130.00


Antibodies against TurboGFP

Anti-TurboGFP
antibody
AB511 Rabbit polyclonal antibody against non-denatured TurboGFP 100 μg € 150.00
AB512 200 μg € 200.00
Anti-TurboGFP(d)
antibody
AB513 Rabbit polyclonal antibody against denatured TurboGFP and CopGFP 100 μg € 150.00
AB514 200 μg € 200.00

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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