Photoactivatable fluorescent proteins

- Light-induced high-contrast photoconversion
- Easy detection by flow cytometry or fluorescence microscopy
- No cofactors, substrates, or chemical staining required
- Suitability for long-term tracking of cellular events

Photoactivatable fluorescent proteins (PAFPs) represent a novel effective tool for monitoring cellular events. PAFPs change spectral properties in response to irradiation with specific light. This provides unique possibilities for the optical labelling and tracking living cells, organelles, and intracellular molecules in a spatio-temporal manner. Moreover, PAFPs open new possibilities to study cell physiology, in particular, they allow careful determination of protein half-life.

Available proteins


PS-CFP2
Monomeric cyan-to-green photoswitchable fluorescent protein:
- up to 2000-fold contrast of photoconversion
- high pH stability


Dendra2
Monomeric green-to-red photoswitchable fluorescent protein:
- up to 4000-fold contrast of photoconversion
- switched by UV and blue light


KFP-Red
Kindling red fluorescent protein:
- fluorescence is induced by green laser irradiation
- can be kindled reversibly or irreversibly

Related products
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The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
Expression/source vectors
pPS-CFP2-C FP801 Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 C-terminus 20 μg € 400.00
pPS-CFP2-N FP802 Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 N-terminus 20 μg € 400.00
pDendra2-C FP821 Mammalian expression vector encoding humanized Dendra2 and allowing its expression and generation of fusions to the Dendra2 C-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments 20 μg € 400.00
pDendra2-N FP822 Mammalian expression vector encoding humanized Dendra2 and allowing its expression and generation of fusions to the Dendra2 N-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments 20 μg € 400.00
pDendra2-B FP823 Bacterial expression vector; source of the Dendra2 coding sequence 20 μg € 400.00
Gateway® Dendra2-At-C entry clone FP824 Gateway® entry clone for generation of fusions to the C-terminus of Dendra2; transfer of the constract encoding Dendra2 or its fusion into Gateway® destination vectors; Dendra2 codon usage is optimized for expression in Arabidopsis 20 μg € 400.00
Gateway® Dendra2-At-N entry clone FP825 Gateway® entry clone for generation of fusions to the N-terminus of Dendra2; transfer of the constract encoding Dendra2 or its fusion into Gateway® destination vectors; Dendra2 codon usage is optimized for expression in Arabidopsis 20 μg € 400.00
pKindling-Red-N FP301 Mammalian expression vector encoding humanized KFP-Red and allowing its expression and generation of fusions to the KFP-Red N-terminus 20 μg € 400.00
pKindling-Red-B FP302 Bacterial expression vector; source of the KFP-Red coding sequence 20 μg € 400.00
pKindling-Red-mito FP401 Mammalian expression vector encoding humanized KFP-Red targeted to mitochondria 20 μg € 400.00
Vector sets
Mito-tracker vector set FPM01 Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito 20 μg each € 700.00
Recombinant proteins
rKFP-Red FP351 Purified recombinant kindling red fluorescent protein 100 μg € 130.00
Antibodies
Anti-Tag(CGY)FP
antibody
AB121 Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP 100 μg € 150.00
AB122 200 μg € 200.00
Anti-Dendra2
antibody
AB821 Rabbit polyclonal antibody against Dendra2 100 μg € 150.00
AB822 200 μg € 200.00
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