Trimmer and Trimmer-Direct cDNA normalization kits

cat.# NK001; NK002

- Acceleration of transcriptome analysis, functional screening, and rare gene discovery
- Rapid and reliable way to remove repeated transcripts from cDNA library
- Equalization of full-length-enriched cDNA before library cloning
- Simple procedure, no physical separation steps

Evrogen offers cDNA normalization kits designed to generate full-length-enriched double stranded (ds) cDNA with equalized concentrations of different transcripts. Both total RNA and polyA+ RNA can be used for cDNA preparation and subsequent normalization. Depending on your particular needs, you can select Trimmer or Trimmer-Direct kit wherein the first allows preparation of normalized cDNA ready for nondirectional cloning and the second – for directional cloning of cDNA library. Alternatively, DSN-normalized cDNA can be used for 454 sequencing.

Starting materials

Trimmer kit allows normalization of cDNA flanked by the same adapter sequences at the both 3'- and 5'- ends. Starting cDNA can be prepared using one of the following cDNA synthesis kits:

- Mint cDNA synthesis kit (Evrogen Cat.# SK001)
- Mint-Universal cDNA synthesis kit, Protocol-I (Evrogen Cat.# SK002)
- SMART^TM PCR cDNA synthesis kit (Clontech Cat.# 634902).

Trimmer-Direct kit is designed for normalization of cDNA flanked by the different adapter sequences at the 3'- and 5'- ends. Starting cDNA must be prepared using one of the following cDNA synthesis kits:

- Mint-Universal cDNA synthesis kit, Protocol-II (Evrogen Cat.# SK002)
- SMART^TM cDNA Library Construction kit (Clontech Cat.# 634901)*
- Creator^TM SMART^TM cDNA Library Construction kit (Clontech Cat.# 634903)*.

Note:

*Please follow instruction provided in Trimmer-Direct kit user manual for cDNA synthesis using SMART^TM kits. Please note that CDS-3M adapter (provided in Trimmer-Direct kit) must be used for cDNA preparation instead of CDS primer included into Clontech kits.

		Typical cDNA normalization result. (A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. GPDH - glyceraldehyde-3-phosphate dehydrogenase; UBC - ubiquitin C; M, 1-kb DNA size markers (SibEnzyme); embr. - embryonic; sm.intest. - small intestine.

Available products

PRODUCT	CAT.#	DESCRIPTION	SIZE	PRICE	USER MANUAL (PDF)
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
Trimmer kit	NK001	Allows normalization of cDNA for subsequent non-directional cloning	10 rxn	€ 600.00
Trimmer-Direct kit	NK002	Allows normalization of cDNA for subsequent directional cloning	10 rxn	€ 750.00

Storage: Lyophilized DSN enzyme should be stored at +4°C, other components should be stored at -20°C

Notice to Purchaser:

Evrogen nucleic acid-related products are intended for research use only. The products are covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License #002.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.