Cell death typing by monitoring caspase 3 activity.
Constructs used: pCasper3-BG vector.
Expression system: L929sAhFas murine fibrosarcoma cells, transient expression.
Detection system: Simultaneous analysis of cell death and caspase-3 activity in L929pCasper3-BG cells was performed by flow cytometry using a triple-laser LSR-II (405, 488 and 635 nm).


Vanlangenakker N, Bertrand MJ, Bogaert P, Vandenabeele P, Vanden Berghe T. TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members. Cell Death Dis. 2011 Nov 17;2:e230 doi: 10.1038/cddis.2011.111 pmid: 22089168	Cell death typing by monitoring caspase 3 activity. Constructs used: pCasper3-BG vector. Expression system: L929sAhFas murine fibrosarcoma cells, transient expression. Detection system: Simultaneous analysis of cell death and caspase-3 activity in L929pCasper3-BG cells was performed by flow cytometry using a triple-laser LSR-II (405, 488 and 635 nm).