Excitation (thin lines) and emission (thick lines) spectra of TagBFP (blue) and TagGFP2 (green) are shown individually. Spectral overlap is filled with gray.
Download Casper3-BG spectra (xls)
| | CHARACTERISTIC | |
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| | FRET donor | TagBFP | | Fluorescence color | blue | | Excitation maximum, nm | 402 | | Emission maximum, nm | 457 | | Brightness, % of EGFP | 99 | | pKa | 2.7 | | FRET acceptor | TagGFP2 | | Fluorescence color | green | | Excitation maximum, nm | 483 | | Emission maximum, nm | 506 | | Brightness, % of EGFP | 105 | | pKa | 5.0 | | Calculated Förster distance R0 | 5.25 | | FRET efficiency E | 0.57 | | Specificity | caspase-3 activity | | Response | elimination of FRET | | Polypeptide length, aa | 490 | | Molecular weight, kDa | 55 |
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