Green-to-red photoswitchable fluorescent protein Dendra2

- Monomer, successful performance in fusions
- Irreversible photoconversion from a green to a red fluorescent form
- High contrast of photoconversion
- Activated by UV-violet and blue light
- Matures at a wide range of temperatures
- Recommended for tracking cell, organelle, and protein movement, and for determination of protein half-life

Dendra is a mutant of the GFP-like protein from octocoral Dendronephthya sp. [Gurskaya et al., 2006]. Compared with Dendra, Dendra2 comprises single A224V substitution, which results in better maturation and a brighter fluorescence both before and after photoswitching. Dendra2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.

Dendra2-At variant: Dendra2-At codon usage is optimized for high expression in Arabidopsis. This variant is available in Gateway® entry clones.