CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Purified recombinant protein behaves as a pure monomer at concentrations of 10 mg/ml as verified by high-performance liquid chromatography (HPLC) * Time to bleach 50% of fluorescent signal brightness.
Molecular weight, kDa	26
Polypeptide length, aa	232
Fluorescence color	red
Excitation maximum, nm	580
Emission maximum, nm	608
Quantum yield	0.19
Extinction coefficient, M^-1cm^-1	94 500
Brightness*	18.0
Brightness, % of EGFP	53
pKa	4.6
Structure	supermonomer**
Aggregation	no
Maturation half-time, min	130
Photostability, widefield***	150
Photostability, confocal***	176
Cell toxicity	not observed
Possible limitations	superior performance in fusions, low cytotoxicity