Main properties

Katushka2S spectra

Katushka2S normalized excitation (thin line) and emission (thick line) spectra.

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CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26
Polypeptide length, aa235
Fluorescence colorfar-red
Excitation maximum, nm588
Emission maximum, nm633
Quantum yield0.44
Extinction coefficient, M-1cm-167 000
Brightness*29.5
Brightness, % of EGFP89
pKa5.4
Structuredimer
Aggregationno
Maturation rate at 37°Csuper fast
Photostability,widefield (sec)72
Cell toxicitynot observed
Main advantagessuper bright and fast-maturing far-red fluorescent protein; high pH-stability
Possible limitationsdimer, limited applicability for fusions generation, medium photostability