Blue line – excitation (em@600 nm),
Orange line – emission (ex@510 nm)
| CHARACTERISTIC | |
|---|---|
| * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. | |
| Molecular weight, kDa | 27 |
| Polypeptide length, aa | 239 |
| Structure | dimer |
| Aggregation | no |
| Maturation rate at 37°C | fast |
| Activating light | blue or green (e.g. 450-540 nm) |
| Fluorescence color | orange |
| Excitation maximum, nm | 512 |
| Emission maximum, nm | 555 |
| Quantum yield | 0.42 |
| Extinction coefficient, M-1cm-1 | 41200 |
| Brightness* | 17.3 |