Normalized excitation (green line) and emission (red line) spectra.
| CHARACTERISTIC | |
|---|---|
| * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. | |
| Molecular weight, kDa | 27 |
| Polypeptide length, aa | 239 |
| Structure | dimer |
| Aggregation | no |
| Maturation rate at 37°C | fast |
| Activating light | green or orange (e.g. 540-590 nm) |
| Fluorescence color | red |
| Excitation maximum, nm | 585 |
| Emission maximum, nm | 610 |
| Quantum yield | 0.25 |
| Extinction coefficient, M-1cm-1 | 45 000 |
| Brightness* | 11.3 |