Far-red fluorescent protein TagFP635

- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling

Available variants and fusions

TagFP635 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. Evrogen mammalian expression vectors comprising multiple cloning sites at the 5'- or 3'-end of TagFP635 coding sequence allow easy generation of fusions of interest.

TagFP635-actinin fusion: Human α-actinin is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-actinin in living cells.

TagFP635-tubulin fusion: Human α-tubulin is fused to the TagFP635 C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of α-tubulin in living cells.

TagFP635-actin fusion: Human β-actin is linked to the TagFP635 C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of β-actin in living cells.

TagFP635-EB3 fusion: Human microtubule-associated EB3 protein is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of EB3 protein in living cells.

TagFP635-vinculin fusion: Human vinculin is fused to the TagFP635 C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vinculin in living cells.

TagFP635-zyxin fusion: Human zyxin is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of zyxin in living cells.

TagFP635-Cx26 fusion: Rat connexin 26 is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 26 in living cells.

TagFP635-Cx32 fusion: Human connexin 32 is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 32 in living cells.

TagFP635-Cx43 fusion: Rat connexin 43 is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 43 in living cells.

TagFP635-H2B fusion: Human histone H2B is fused to the TagFP635 N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells.

TagFP635-laminB1 fusion: Human lamin B1 is fused to the TagFP635 C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of lamin B1 in living cells.