
Typical cDNA normalization result. (A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. GPDH – glyceraldehyde-3-phosphate dehydrogenase; UBC – ubiquitin C; M, 1-kb DNA size markers (SibEnzyme). (C) Normalization significantly increases gene discovery rate of cDNA library. Transcript distribution in standard and normalized cDNA libraries from Aplysia neurons: yellow – all sequences, blue – unique sequences; green – non-unique sequences.

Typical cDNA normalization result.

(A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. GPDH – glyceraldehyde-3-phosphate dehydrogenase; UBC – ubiquitin C; M, 1-kb DNA size markers (SibEnzyme). (C) Normalization significantly increases gene discovery rate of cDNA library. Transcript distribution in standard and normalized cDNA libraries from Aplysia neurons: yellow – all sequences, blue – unique sequences; green – non-unique sequences.