Main properties

TurboRFP spectra

TurboRFP normalized excitation (thin line) and emission (thick line) spectra.

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CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26.1
Polypeptide length, aa231
Fluorescence colorred (orange)
Excitation maximum, nm553
Emission maximum, nm574
Quantum yield0.67
Extinction coefficient, M-1cm-192 000
Brightness*61.6
Brightness, % of EGFP187
pKa4.4
Structuredimer
Aggregationno
Maturation rate at 37°Csuper fast
Photostabilityhigh
Cell toxicitynot observed
Main advantagessuperbright and fast-maturing red fluorescent protein
Possible limitationsdimer, limited applicability for fusions generation