CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000. ** Time to bleach 50% of fluorescent signal brightness. Extinction coefficient and quantum yield were measured at the physiological pH=7.5.
Molecular weight, kDa	26
Polypeptide length, aa	232
Fluorescence color	far-red
Excitation maximum, nm	588
Emission maximum, nm	633
Quantum yield	0.40
Extinction coefficient, M^-1cm^-1	62 500
Brightness*	25.0
Brightness, % of EGFP	74
pKa	5.4
Structure	monomer
Aggregation	no
Maturation half-time, min	<20
Photostability, widefield**	69
Photostability, confocal**	390
Cell toxicity	not observed
Main advantages	bright monomeric far-red fluorescent protein, high pH- and photostability