Available variants and fusions

Variant	Description	Related vector	Cat.#	Click for image
Humanized mKate2	mKate2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.	pmKate2-C	FP181
		pmKate2-N	FP182
mKate2-actin fusion	Human β-actin is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of β-actin in living cells.	pmKate2-actin	FP184
mKate2-f-mem fusion	20 amino acid farnesylation signal from c-Ha-Ras is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of plasma membrane.	pmKate2-f-mem	FP186
mKate2-mito fusion	A mitochondrial targeting sequence (MTS) is fused to the mKate2 N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides far-red fluorescent labeling of mitochondria.	pmKate2-mito	FP187
mKate2-H2B fusion	Human histone H2B is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of histone H2B in living cells.	pmKate2-H2B	FP311
mKate2-lyso fusion	Rat Lysosomal Associated Membrane Protein 1 (LAMP-1) is fused to the mKate2 N-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of lysosomes.	pmKate2-lyso	FP312
mKate2-peroxi fusion	Peroxisomal targeting signal [Gould et al., 1989] encoding tripeptide SKL is fused to the 3' end of mKate2 sequence. This tripeptide targets the fusion protein to the matrix of peroxisomes.	pmKate2-peroxi	FP313
mKate2-endo fusion	Human RhoB GTPase is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of vesicles of the endocytic pathway.	pmKate2-endo	FP314
mKate2-clathrin fusion	Human clathrin LCB is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of clathrin LCB in living cells.	pmKate2-clathrin	FP322
mKate2-ER fusion	Signal sequence of calreticulin is fused to the mKate2 N-terminus and the endoplasmic reticulum retention sequence KDEL is fused to the mKate2 C-terminus. When expressed in mammalian cells, this fusion provides far-red fluorescent labeling of the lumen of the endoplasmic reticulum.	pmKate2-ER	FP324