Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Case12
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1924-1926
Last amino acid in Case12: 1921-1923
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1922-1927, 2080-2085 & 2109-2114
mRNA 3' ends: 2118 & 2130
f1 single-strand DNA origin: 2177-2632
Bacterial promoter for expression of Kan^r gene
-35 region: 2694-2699
-10 region: 2717-2722
Transcription start point: 2729
SV40 origin of replication: 2973-3108
SV40 early promoter
Enhancer (72-bp tandem repeats): 2806-2877 & 2878-2949
21-bp repeats: 2953-2973, 2974-2994 & 2996-3016
Early promoter element: 3029-3035
Major transcription start points: 3025, 3063, 3069 & 3074
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3157-3159
Stop codon: 3949-3951
G->A mutation to remove Pst I site: 3339
C->A (Arg to Ser) mutation to remove BssH II site: 3685
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4187-4192 & 4200-4205
pUC plasmid replication origin: 4536-5179