Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Case12-Mito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal (MLS): 597-683
Start of Case12 coding sequence (ATG): 705-707
Stop codon: 1950-1952
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1948-1953, 2106-2111 & 2135-2140
mRNA 3' ends: 2144 & 2156
f1 single-strand DNA origin: 2203-2658
Bacterial promoter for expression of Kan^r gene
-35 region: 2720-2725
-10 region: 2743-2748
Transcription start point: 2755
SV40 origin of replication: 2999-3134
SV40 early promoter
Enhancer (72-bp tandem repeats): 2832-2903 & 2904-2975
21-bp repeats: 2979-2999, 3000-3020 & 3022-3042
Early promoter element: 3055-3061
Major transcription start points: 3051, 3089, 3095 & 3100
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3183-3185
Stop codon: 3975-3977
G->A mutation to remove Pst I site: 3365
C->A (Arg to Ser) mutation to remove BssH II site: 3711
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4213-4218 & 4226-4231
pUC plasmid replication origin: 4562-5205