RESEARCH PRODUCTS/Photoactivatable fluorescent proteins/Dendra2


    pDendra2-C

pDendra2-C vector

cat.# FP821

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
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pDendra2-CFP82120 μg€ 400.00
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Vector typemammalian expression vector
ReporterDendra2
Reporter codon usagemammalian
Promoter for Dendra2PCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use Dendra2 expression in mammalian cells; generation of fusions to the Dendra2 C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Dendra2
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1393-1395
Last amino acid in Dendra: 1300-1302
MCS: 1327-1393
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1535-1540 & 1564-1569
mRNA 3' ends: 1573 & 1585
f1 single-strand DNA origin: 1632-2087
Eukaryotic promoter for expression of Kanr gene
-35 region: 2149-2154
-10 region: 2172-2177
Transcription start point: 2184
SV40 origin of replication: 2428-2563
SV40 early promoter
Enhancer (72-bp tandem repeats): 2261-2332 & 2333-2404
21-bp repeats: 2408-2428, 2429-2449 & 2451-2471
Early promoter element: 2484-2490
Major transcription start points: 2480, 2518, 2524 & 2529
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2612-2614
Stop codon: 3404-3406
G->A mutation to remove Pst I site: 2794
C->A (Arg to Ser) mutation to remove BssH II site: 3140
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3642-3647 & 3655-3660
pUC plasmid replication origin: 3991-4634


Notice to Purchaser:

Photoactivatable FP-related products are intended to be used by academic (non-commercial) entities and for research purposes only. Any use of the proprietary nucleic acid or protein other than for research use or by a commercial entity is strictly prohibited. Transfer of this product by purchaser to any other party is specifically prohibited.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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