Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
FusionRed
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
Farnesylation signal: 1336-1398
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1590-1595 & 1306-1624
mRNA 3' ends: 1628 & 1640
f1 single-strand DNA origin: 1687-2142
Bacterial promoter for expression of Kan^r gene
-35 region: 2204-2209
-10 region: 2227-2232
Transcription start point: 2239
SV40 origin of replication: 2483-2618
SV40 early promoter
Enhancer (72-bp tandem repeats): 2316-2387 & 2388-2459
21-bp repeats: 2463-2483, 2484-2504 & 2506-2526
Early promoter element: 2539-2545
Major transcription start points: 2535, 2573, 2579 & 2584
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2667-2669
Stop codon: 3459-3461
G->A mutation to remove Pst I site: 2849
C->A (Arg to Ser) mutation to remove BssH II site: 3195
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3697-3702 & 3710-3715
pUC plasmid replication origin: 4046-4689