Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Talin-FusionRed fusion: 643-9030
Talin: 643-8265
Start codon (ATG): 643-645
Last amino acid in Talin: 8263-8265
FusionRed: 8332-9030
Stop codon: 9028-9030
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 9183-9188 & 9212-9217
mRNA 3' ends: 9221 & 9233
f1 single-strand DNA origin: 9280-9735
Bacterial promoter for expression of Kan^r gene
-35 region: 9797-9802
-10 region: 9820-9825
Transcription start point: 9832
SV40 origin of replication: 10076-10211
SV40 early promoter
Enhancer (72-bp tandem repeats): 9909-9980 & 9981-10052
21-bp repeats: 10056-10076, 10077-10097 & 10099-10119
Early promoter element: 10132-10138
Major transcription start points: 10128, 10166, 10172 & 10177
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 10260-10262
Stop codon: 11052-11054
G->A mutation to remove Pst I site: 10442
C->A (Arg to Ser) mutation to remove BssH II site: 10788
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 11290-11295 & 11303-11308
pUC plasmid replication origin: 11639-12282