Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
HyPer-nuc fusion
Start codon (ATG): 603-605
HyPer coding sequence: 603-2036
Nuclear localization signals (NLS): 2058-2130
Stop codon: 2148-2150
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2290-2295 & 2319-2324
mRNA 3' ends: 2328 & 2340
f1 single-strand DNA origin: 2387-2842
Bacterial promoter for expression of Kan^r gene
-35 region: 2904-2909
-10 region: 2927-2932
Transcription start point: 2939
SV40 origin of replication: 3183-3318
SV40 early promoter
Enhancer (72-bp tandem repeats): 3016-3087 & 3088-3159
21-bp repeats: 3163-3183, 3184-3204 & 3206-3226
Early promoter element: 3239-3245
Major transcription start points: 3235, 3273, 3279 & 3284
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3367-3369
Stop codon: 4159-4161
G->A mutation to remove Pst I site: 3549
C->A (Arg to Ser) mutation to remove BssH II site: 3895
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4397-4402 & 4410-4415
pUC plasmid replication origin: 4746-5389