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RESEARCH PRODUCTS/Basic fluorescent proteins/JRed

pJRed-C

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pJRed-C vector

cat.# FP701

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pJRed-C	FP701	20 μg	€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector type	mammalian expression vector
Reporter	JRed
Reporter codon usage	mammalian
Promoter for JRed	P_{CMV IE}
Host cells	mammalian
Selection	prokaryotic - kanamycin eukaryotic - neomycin (G418)
Replication	prokaryotic - pUC ori eukaryotic - SV40 ori
Use	JRed expression in mammalian cells; generation of fusions to the JRed C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
JRed
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1417-1419
Last amino acid in JRed: 1336-1338
MCS: 1339-1424
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1559-1564 & 1588-1593
mRNA 3' ends: 1597 & 1609
f1 single-strand DNA origin: 1656-2111
Eukaryotic promoter for expression of Kan^r gene
-35 region: 2173-2178
-10 region: 2196-2201
Transcription start point: 2208
SV40 origin of replication: 2452-2587
SV40 early promoter
Enhancer (72-bp tandem repeats): 2285-2356 & 2357-2428
21-bp repeats: 2432-2452, 2453-2473 & 2475-2495
Early promoter element: 2508-2514
Major transcription start points: 2504, 2542, 2548 & 2553
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2636-2638
Stop codon: 3428-3430
G->A mutation to remove Pst I site: 2818
C->A (Arg to Ser) mutation to remove BssH II site: 3164
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3666-3671 & 3679-3684
pUC plasmid replication origin: 4015-4658

Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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