RESEARCH PRODUCTS/Basic fluorescent proteins/JRed


    pJRed-N

pJRed-N vector

cat.# FP702

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download
vector information:
ProductCat.#SizePrice
pJRed-NFP70220 μg€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterJRed
Reporter codon usagemammalian
Promoter for JRedPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use JRed expression in mammalian cells; generation of fusions to the JRed N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
JRed
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1405-1407
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1561-1566 & 1590-1595
mRNA 3' ends: 1599 & 1611
f1 single-strand DNA origin: 1658-2113
Eukaryotic promoter for expression of Kanr gene
-35 region: 2175-2180
-10 region: 2198-2203
Transcription start point: 2210
SV40 origin of replication: 2454-2589
SV40 early promoter
Enhancer (72-bp tandem repeats): 2287-2358 & 2359-2430
21-bp repeats: 2434-2454, 2455-2475 & 2477-2497
Early promoter element: 2510-2516
Major transcription start points: 2506, 2544, 2550 & 2555
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2638-2640
Stop codon: 3430-3432
G->A mutation to remove Pst I site: 2820
C->A (Arg to Ser) mutation to remove BssH II site: 3166
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3668-3673 & 3681-3686
pUC plasmid replication origin: 4017-4660


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

Copyright 2002-2008 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com