pJRed-PRL vector

cat.# FP705

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pJRed-PRL FP705 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter JRed Reporter codon usage mammalian Promoter for JRed NO Host cells mammalian, prokaryotic Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use Monitoring transcription from different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
JRed
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 823-825
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 979-984 & 1008-1013
mRNA 3' ends: 1017 & 1029
f1 single-strand DNA origin: 1076-1531
Eukaryotic promoter for expression of Kan^r gene
-35 region: 1593-1598
-10 region: 1616-1621
Transcription start point: 1628
SV40 origin of replication: 1872-2007
SV40 early promoter
Enhancer (72-bp tandem repeats): 1705-1776 & 1777-1848
21-bp repeats: 1852-1872, 1873-1893 & 1895-1915
Early promoter element: 1928-1934
Major transcription start points: 1924, 1962, 1968 & 1973
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2056-2058
Stop codon: 2848-2850
G->A mutation to remove Pst I site: 2238
C->A (Arg to Ser) mutation to remove BssH II site: 2584
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3086-3091 & 3099-3104
pUC plasmid replication origin: 3435-4078