Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
KFP-Red
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1530-1535 & 1559-1564
mRNA 3' ends: 1568 & 1580
f1 single-strand DNA origin: 1627-2082
Bacterial promoter for expression of Kan^r gene
-35 region: 2144-2149
-10 region: 2167-2172
Transcription start point: 2179
SV40 origin of replication: 2423-2558
SV40 early promoter
Enhancer (72-bp tandem repeats): 2256-2327 & 2328-2399
21-bp repeats: 2403-2423, 2424-2444 & 2446-2466
Early promoter element: 2479-2485
Major transcription start points: 2475, 2513, 2519 & 2524
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2607-2609
Stop codon: 3399-3401
G->A mutation to remove Pst I site: 2789
C->A (Arg to Ser) mutation to remove BssH II site: 3135
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3637-3642 & 3650-3655
pUC plasmid replication origin: 3986-4629