pKatushka2S-B is a prokaryotic expression vector encoding far-red fluorescent protein Katushka2S (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of Katushka2S coding sequence. Flanking restriction sites are convenient for excision of Katushka2S sequence and its further insertion into other expression vectors of choice. Alternatively, Katushka2S coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam⁺-methylated E. coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam^- host and make fresh DNA.

The vector can be also used for Katushka2S expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.