Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-678
KillerOrange
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1390-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1545-1550 & 1574-1579
mRNA 3' ends: 1583 & 1595
f1 single-strand DNA origin: 1642-2097
Bacterial promoter for expression of Kan^r gene
-35 region: 2159-2164
-10 region: 2182-2187
Transcription start point: 2194
SV40 origin of replication: 2438-2573
SV40 early promoter
Enhancer (72-bp tandem repeats): 2271-2342 & 2343-2414
21-bp repeats: 2418-2438, 2439-2459 & 2461-2481
Early promoter element: 2494-2500
Major transcription start points: 2490, 2528, 2534 & 2539
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2622-2624
Stop codon: 3414-3416
G->A mutation to remove Pst I site: 2804
C->A (Arg to Ser) mutation to remove BssH II site: 3150
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3652-3657 & 3665-3670
pUC plasmid replication origin: 4001-4644