Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KillerOrange-dMito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal 1 (MLS-1): 597-689
Mitochondrial localization signal 2 (MLS-2): 690-782
Start of KillerOrange coding sequence(GAG): 801-803
Stop codon: 1509-1511
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1664-1669 & 1693-1698
mRNA 3' ends: 1702 & 1714
f1 single-strand DNA origin: 1761-2216
Bacterial promoter for expression of Kan^r gene
-35 region: 2278-2283
-10 region: 2301-2306
Transcription start point: 2313
SV40 origin of replication: 2557-2692
SV40 early promoter
Enhancer (72-bp tandem repeats): 2390-2461 & 2462-2533
21-bp repeats: 2537-2557, 2558-2578 & 2580-2600
Early promoter element: 2613-2619
Major transcription start points: 2609, 2647, 2653 & 2658
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2741-2743
Stop codon: 3533-3535
G->A mutation to remove Pst I site: 2923
C->A (Arg to Ser) mutation to remove BssH II site: 3269
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3771-3776 & 3784-3789
pUC plasmid replication origin: 4120-4763