RESEARCH PRODUCTS/KillerRed


    pKillerRed-dMito

SEARCH

pKillerRed-dMito vector

cat.# FP964

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download
vector information:
ProductCat.#SizePrice
pKillerRed-dMitoFP96420 μg€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterKillerRed
Reporter codon usagemammalian
Promoter for KillerRedPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use Expression of mitochondria-targeted KillerRed in mammalian cells under the control of CMV promoter; source of mitochondria-targeted KillerRed coding sequence

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KillerRed-dMito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal 1 (MLS-1): 597-689
Mitochondrial localization signal 2 (MLS-2): 690-782
Start of KillerRed coding sequence (ATG): 798-800
Stop codon: 1515-1517
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1671-1676 & 1700-1705
mRNA 3' ends: 1709 & 1721
f1 single-strand DNA origin: 1768-2223
Eukaryotic promoter for expression of Kanr gene
-35 region: 2285-2290
-10 region: 2308-2313
Transcription start point: 2320
SV40 origin of replication: 2564-2699
SV40 early promoter
Enhancer (72-bp tandem repeats): 2397-2468 & 2469-2540
21-bp repeats: 2544-2564, 2565-2585 & 2587-2607
Early promoter element: 2620-2626
Major transcription start points: 2616, 2654, 2660 & 2665
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2748-2750
Stop codon: 3540-3542
G->A mutation to remove Pst I site: 2930
C->A (Arg to Ser) mutation to remove BssH II site: 3276
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3778-3783 & 3791-3796
pUC plasmid replication origin: 4127-4770


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

Copyright 2002-2008 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com