Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PS-CFP2
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1393-1395
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1549-1554 & 1578-1583
mRNA 3' ends: 1587 & 1599
f1 single-strand DNA origin: 1646-2101
Bacterial promoter for expression of Kan^r gene
-35 region: 2163-2168
-10 region: 2186-2191
Transcription start point: 2198
SV40 origin of replication: 2442-2577
SV40 early promoter
Enhancer (72-bp tandem repeats): 2275-2346 & 2347-2418
21-bp repeats: 2422-2442, 2443-2463 & 2465-2485
Early promoter element: 2498-2504
Major transcription start points: 2494, 2532, 2538 & 2543
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2626-2628
Stop codon: 3418-3420
G->A mutation to remove Pst I site: 2808
C->A (Arg to Ser) mutation to remove BssH II site: 3154
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3656-3661 & 3669-3674
pUC plasmid replication origin: 4005-4648