Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PhiYFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1597-1602 & 1626-1631
mRNA 3' ends: 1635 & 1647
f1 single-strand DNA origin: 1694-2149
Bacterial promoter for expression of Kan^r gene
-35 region: 2211-2216
-10 region: 2234-2239
Transcription start point: 2246
SV40 origin of replication: 2490-2625
SV40 early promoter
Enhancer (72-bp tandem repeats): 2323-2394 & 2395-2466
21-bp repeats: 2470-2490, 2491-2511 & 2513-2533
Early promoter element: 2546-2552
Major transcription start points: 2542, 2580, 2586 & 2591
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2674-2676
Stop codon: 3466-3468
G->A mutation to remove Pst I site: 2856
C->A (Arg to Ser) mutation to remove BssH II site: 3202
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3704-3709 & 3717-3722
pUC plasmid replication origin: 4053-4696