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    pPhi-Yellow-PRL-dest1

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pPhi-Yellow-PRL-dest1 vector

cat.# FP605

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pPhi-Yellow-PRL-dest1FP60520 μg€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typepromoterless expression vector
ReporterPhiYFP
Reporter codon usagemammalian
Promoter for PhiYFPNO
Host cellsmammalian, prokaryotic
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use Monitoring transcription from different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
PhiYFP-m-dest1
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Last amino acid in XGFP: 796-798
Stop codon: 934-936
MODC PEST sequence: 814-936
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1091-1096 & 1120-1125
mRNA 3' ends: 1129 & 1141
f1 single-strand DNA origin: 1188-1643
Eukaryotic promoter for expression of Kanr gene
-35 region: 1705-1710
-10 region: 1728-1733
Transcription start point: 1740
SV40 origin of replication: 1984-2119
SV40 early promoter
Enhancer (72-bp tandem repeats): 1817-1888 & 1889-1960
21-bp repeats: 1964-1984, 1985-2005 & 2007-2027
Early promoter element: 2040-2046
Major transcription start points: 2036, 2074, 2080 & 2085
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2168-2170
Stop codon: 2960-2962
G->A mutation to remove Pst I site: 2350
C->A (Arg to Ser) mutation to remove BssH II site: 2696
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3198-3203 & 3211-3216
pUC plasmid replication origin: 3547-4190


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