Location of features

MCS: 12-89
PhiYFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 799-801
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1015-1020 & 1044-1049
mRNA 3' ends: 1053 & 1065
f1 single-strand DNA origin: 1112-1567
Bacterial promoter for expression of Kan^r gene
-35 region: 1629-1634
-10 region: 1652-1657
Transcription start point: 1664
SV40 origin of replication: 1908-2043
SV40 early promoter
Enhancer (72-bp tandem repeats): 1741-1812 & 1813-1884
21-bp repeats: 1888-1908, 1909-1929 & 1931-1951
Early promoter element: 1964-1970
Major transcription start points: 1960, 1998, 2004 & 2009
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2092-2094
Stop codon: 2884-2886
G->A mutation to remove Pst I site: 2274
C->A (Arg to Ser) mutation to remove BssH II site: 2620
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3122-3127 & 3135-3140
pUC plasmid replication origin: 3471-4114