RESEARCH PRODUCTS/Basic fluorescent proteins/PhiYFP


    pPhi-Yellow-dest1

pPhi-Yellow-dest1 vector

cat.# FP608

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pPhi-Yellow-dest1FP60820 μg€ 400.00
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Vector typemammalian expression vector
ReporterPhiYFP
Reporter codon usagemammalian
Promoter for PhiYFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use PhiYFP expression in mammalian cells; generation of fusions to the PhiYFP-dest1 N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PhiYFP-m
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in PhiYFP-m: 1378-1380
Stop codon: 1516-1518
MODC PEST sequence: 1396-1518
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1673-1678 & 1702-1707
mRNA 3' ends: 1711 & 1723
f1 single-strand DNA origin: 1770-2225
Eukaryotic promoter for expression of Kanr gene
-35 region: 2287-2292
-10 region: 2310-2315
Transcription start point: 2322
SV40 origin of replication: 2566-2701
SV40 early promoter
Enhancer (72-bp tandem repeats): 2399-2470 & 2471-2542
21-bp repeats: 2546-2566, 2567-2587 & 2589-2609
Early promoter element: 2622-2628
Major transcription start points: 2618, 2656, 2662 & 2667
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2750-2752
Stop codon: 3542-3544
G->A mutation to remove Pst I site: 2932
C->A (Arg to Ser) mutation to remove BssH II site: 3278
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3780-3785 & 3793-3798
pUC plasmid replication origin: 4129-4772


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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