Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 592-678
BrUSLEE
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1551-1556 & 1580-1585
mRNA 3' ends: 1589 & 1601
f1 single-strand DNA origin: 1648-2103
Bacterial promoter for expression of Kan^r gene
-35 region: 2165-2170
-10 region: 2188-2193
Transcription start point: 2200
SV40 origin of replication: 2444-2579
SV40 early promoter
Enhancer (72-bp tandem repeats): 2277-2348 & 2349-2420
21-bp repeats: 2424-2444, 2445-2465 & 2467-2487
Early promoter element: 2500-2506
Major transcription start points: 2496, 2534, 2540 & 2545
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2628-2630
Stop codon: 3420-3422
G->A mutation to remove Pst I site: 2810
C->A (Arg to Ser) mutation to remove BssH II site: 3156
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3658-3663 & 3671-3676
pUC plasmid replication origin: 4007-4650