pTagBFP-actin vector

cat.# FP174

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagBFP-actin FP174 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagBFP Reporter codon usage mammalian Promoter for TagBFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use blue fluorescent labeling of β-actin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagBFP- beta-Actin fusion: 607-2454
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609
Last amino acid in TagBFP: 1303-1305
beta-Actin: 1327-2454
Stop codon: 2452-2454
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2615-2620 & 2644-2649
mRNA 3' ends: 2653 & 2665
f1 single-strand DNA origin: 2712-3167
Bacterial promoter for expression of Kan^r gene
-35 region: 3229-3234
-10 region: 3252-3257
Transcription start point: 3264
SV40 origin of replication: 3508-3643
SV40 early promoter
Enhancer (72-bp tandem repeats): 3341-3412 & 3413-3484
21-bp repeats: 3488-3508, 3509-3529 & 3531-3551
Early promoter element: 3564-3570
Major transcription start points: 3560, 3598, 3604 & 3609
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3692-3694
Stop codon: 4484-4486
G->A mutation to remove Pst I site: 3874
C->A (Arg to Ser) mutation to remove BssH II site: 4220
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4722-4727 & 4735-4740
pUC plasmid replication origin: 5071-5714