RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs/TagFP635


    pTagFP635-C

pTagFP635-C vector

cat.# FP161

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
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pTagFP635-CFP16120 μg€ 400.00
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Vector typemammalian expression vector
ReporterTagFP635
Reporter codon usagemammalian
Promoter for TagFP635PCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use TagFP635 expression in mammalian cells; generation of fusions to the TagFP635 C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagFP635
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1401-1403
Last amino acid in TurboGFP: 1321-1323
MCS: 1324-1403
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1543-1548 & 1572-1577
mRNA 3' ends: 1581 & 1593
f1 single-strand DNA origin: 1640-2095
Eukaryotic promoter for expression of Kanr gene
-35 region: 2157-2162
-10 region: 2180-2185
Transcription start point: 2192
SV40 origin of replication: 2436-2571
SV40 early promoter
Enhancer (72-bp tandem repeats): 2269-2340 & 2341-2412
21-bp repeats: 2416-2436, 2437-2457 & 2459-2479
Early promoter element: 2492-2498
Major transcription start points: 2488, 2526, 2532 & 2537
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2620-2622
Stop codon: 3412-3414
G->A mutation to remove Pst I site: 2802
C->A (Arg to Ser) mutation to remove BssH II site: 3148
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3650-3655 & 3663-3668
pUC plasmid replication origin: 3999-4642


Notice to Purchaser:

Evrogen FP-related products are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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