pTagFP635-Cx26 vector

cat.# FP382

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagFP635-Cx26 FP382 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagFP635 Reporter codon usage mammalian Promoter for TagFP635 PCMV IE Host cells mammalian Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use far-red fluorescent labeling of connexin 26

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 26: 683-1360
TagFP635: 1382-2095
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2248-2253 & 2277-2282
mRNA 3' ends: 2286 & 2298
f1 single-strand DNA origin: 2345-2800
Bacterial promoter for expression of Kan^r gene
-35 region: 2862-2867
-10 region: 2885-2890
Transcription start point: 2897
SV40 origin of replication: 3141-3276
SV40 early promoter
Enhancer (72-bp tandem repeats): 2974-3045 & 3046-3117
21-bp repeats: 3121-3141, 3142-3162 & 3164-3184
Early promoter element: 3197-3203
Major transcription start points: 3193, 3231, 3237 & 3242
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3325-3327
Stop codon: 4117-4119
G->A mutation to remove Pst I site: 3507
C->A (Arg to Ser) mutation to remove BssH II site: 3853
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4355-4360 & 4368-4373
pUC plasmid replication origin: 4704-5347