pTagFP635-Cx32 vector

cat.# FP383

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagFP635-Cx32 FP383 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagFP635 Reporter codon usage mammalian Promoter for TagFP635 PCMV IE Host cells mammalian Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use far-red fluorescent labeling of connexin 32

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 32: 697-1545
TagFP635: 1567-2280
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2433-2438 & 2462-2467
mRNA 3' ends: 2471 & 2483
f1 single-strand DNA origin: 2530-2985
Bacterial promoter for expression of Kan^r gene
-35 region: 3047-3052
-10 region: 3070-3075
Transcription start point: 3082
SV40 origin of replication: 3326-3461
SV40 early promoter
Enhancer (72-bp tandem repeats): 3159-3230 & 3231-3302
21-bp repeats: 3306-3326, 3327-3347 & 3349-3369
Early promoter element: 3382-3388
Major transcription start points: 3378, 3416, 3422 & 3427
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3510-3512
Stop codon: 4302-4304
G->A mutation to remove Pst I site: 3692
C->A (Arg to Ser) mutation to remove BssH II site: 4038
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4540-4545 & 4553-4558
pUC plasmid replication origin: 4889-5532