pTagFP635-Cx43 vector

cat.# FP384

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagFP635-Cx43 FP384 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagFP635 Reporter codon usage mammalian Promoter for TagFP635 PCMV IE Host cells mammalian Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use far-red fluorescent labeling of connexin 43

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 43: 824-1969
TagFP635: 1991-2704
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2857-2862 & 2886-2891
mRNA 3' ends: 2895 & 2907
f1 single-strand DNA origin: 2954-3409
Bacterial promoter for expression of Kan^r gene
-35 region: 3471-3476
-10 region: 3494-3499
Transcription start point: 3506
SV40 origin of replication: 3750-3885
SV40 early promoter
Enhancer (72-bp tandem repeats): 3583-3654 & 3655-3726
21-bp repeats: 3730-3750, 3751-3771 & 3773-3793
Early promoter element: 3806-3812
Major transcription start points: 3802, 3840, 3846 & 3851
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3934-3936
Stop codon: 4726-4728
G->A mutation to remove Pst I site: 4116
C->A (Arg to Ser) mutation to remove BssH II site: 4462
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4964-4969 & 4977-4982
pUC plasmid replication origin: 5313-5956