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    pTagFP635-laminB1

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pTagFP635-laminB1 vector

cat.# FP387

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pTagFP635-laminB1FP38720 μg€ 400.00
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Vector typemammalian expression vector
ReporterTagFP635
Reporter codon usagemammalian
Promoter for TagFP635PCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use far-red fluorescent labeling of lamin B1

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagFP635
Start codon (ATG): 613-615
Last amino acid in TagFP635: 1321-1323
Lamin B1: 1354-3114
Stop codon: 3112-3114
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 3275-3280 & 3304-3309
mRNA 3' ends: 3313 & 3325
f1 single-strand DNA origin: 3372-3827
Bacterial promoter for expression of Kanr gene
-35 region: 3889-3894
-10 region: 3912-3917
Transcription start point: 3924
SV40 origin of replication: 4168-4303
SV40 early promoter
Enhancer (72-bp tandem repeats): 4001-4072 & 4073-4144
21-bp repeats: 4148-4168, 4169-4189 & 4191-4211
Early promoter element: 4224-4230
Major transcription start points: 4220, 4258, 4264 & 4269
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 4352-4354
Stop codon: 5144-5146
G->A mutation to remove Pst I site: 4534
C->A (Arg to Ser) mutation to remove BssH II site: 4880
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 5382-5387 & 5395-5400
pUC plasmid replication origin: 5731-6374


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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