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    pTagGFP-C

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pTagGFP-C vector

cat.# FP121

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
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pTagGFP-CFP12120 μg€ 400.00
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Vector typemammalian expression vector
ReporterTagGFP
Reporter codon usagemammalian
Promoter for TagGFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use TagGFP expression in mammalian cells; generation of fusions to the TagGFP C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagGFP
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1405-1407
Last amino acid in TagGFP: 1324-1326
MCS: 1327-1404
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1547-1552 & 1576-1581
mRNA 3' ends: 1585 & 1597
f1 single-strand DNA origin: 1644-2099
Eukaryotic promoter for expression of Kanr gene
-35 region: 2161-2166
-10 region: 2184-2189
Transcription start point: 2196
SV40 origin of replication: 2440-2575
SV40 early promoter
Enhancer (72-bp tandem repeats): 2273-2344 & 2345-2416
21-bp repeats: 2420-2440, 2441-2461 & 2463-2483
Early promoter element: 2496-2502
Major transcription start points: 2492, 2530, 2536 & 2541
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2624-2626
Stop codon: 3416-3418
G->A mutation to remove Pst I site: 2806
C->A (Arg to Ser) mutation to remove BssH II site: 3152
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3654-3659 & 3667-3672
pUC plasmid replication origin: 4003-4646


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only. The Products are covered by U.S. Pat. # 7,417,131 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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