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RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs/TagGFP

pTagGFP-tubulin

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pTagGFP-tubulin vector

cat.# FP125

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pTagGFP-tubulin	FP125	20 μg	€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector type	mammalian expression vector
Reporter	TagGFP
Reporter codon usage	mammalian
Promoter for TagGFP	P_{CMV IE}
Host cells	mammalian
Selection	prokaryotic - kanamycin eukaryotic - neomycin (G418)
Replication	prokaryotic - pUC ori eukaryotic - SV40 ori
Use	green fluorescent labeling of α-tubulin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagGFP
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 2698-2700
Last amino acid in TagGFP: 1324-1326
Tubulin: 1345-2700
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2861-2866 & 2890-2895
mRNA 3' ends: 2899 & 2911
f1 single-strand DNA origin: 2958-3413
Eukaryotic promoter for expression of Kan^r gene
-35 region: 3475-3480
-10 region: 3498-3503
Transcription start point: 3510
SV40 origin of replication: 3754-3889
SV40 early promoter
Enhancer (72-bp tandem repeats): 3587-3658 & 3659-3730
21-bp repeats: 3734-3754, 3755-3775 & 3777-3797
Early promoter element: 3810-3816
Major transcription start points: 3806, 3844, 3850 & 3855
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3938-3940
Stop codon: 4730-4732
G->A mutation to remove Pst I site: 4120
C->A (Arg to Ser) mutation to remove BssH II site: 4466
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4968-4973 & 4981-4986
pUC plasmid replication origin: 5317-5960

Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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