RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs/TagRFP


    pTagRFP-actin

pTagRFP-actin vector

cat.# FP144

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pTagRFP-actinFP14420 μg€ 400.00
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Vector typemammalian expression vector
ReporterTagRFP
Reporter codon usagemammalian
Promoter for TagRFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use red (orange) fluorescent labeling of β-actin filaments

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagRFP
Start codon (ATG): 613-615
Last amino acid in TagRFP: 1321-1323
Beta-Actin: 1345-2472
Stop codon: 2470-2472
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2633-2638 & 2662-2667
mRNA 3' ends: 2671 & 2683
f1 single-strand DNA origin: 2730-3185
Eukaryotic promoter for expression of Kanr gene
-35 region: 3247-3252
-10 region: 3270-3275
Transcription start point: 3282
SV40 origin of replication: 3526-3661
SV40 early promoter
Enhancer (72-bp tandem repeats): 3359-3430 & 3431-3502
21-bp repeats: 3506-3526, 3527-3547 & 3549-3569
Early promoter element: 3582-3588
Major transcription start points: 3578, 3616, 3622 & 3627
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3710-3712
Stop codon: 4502-4504
G->A mutation to remove Pst I site: 3892
C->A (Arg to Ser) mutation to remove BssH II site: 4238
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4740-4745 & 4753-4758
pUC plasmid replication origin: 5089-5732


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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