pTagRFP-integrin vector

cat.# FP361

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagRFP-integrin FP361 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagRFP Reporter codon usage mammalian Promoter for TagRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red (orange) fluorescent labeling of α-V-integrin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Alpha-V-Integrin: 613-3756
TagRFP: 3832-4545
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 4698-4703 & 4727-4732
mRNA 3' ends: 4736 & 4748
f1 single-strand DNA origin: 4795-5250
Bacterial promoter for expression of Kan^r gene
-35 region: 5312-5317
-10 region: 5335-5340
Transcription start point: 5347
SV40 origin of replication: 5591-5726
SV40 early promoter
Enhancer (72-bp tandem repeats): 5424-5495 & 5496-5567
21-bp repeats: 5571-5591, 5592-5612 & 5614-5634
Early promoter element: 5647-5653
Major transcription start points: 5643, 5681, 5687 & 5692
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 5775-5777
Stop codon: 6567-6569
G->A mutation to remove Pst I site: 5957
C->A (Arg to Ser) mutation to remove BssH II site: 6303
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 6805-6810 & 6818-6823
pUC plasmid replication origin: 7154-7797