pTagRFP-keratin vector

cat.# FP369

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagRFP-keratin FP369 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagRFP Reporter codon usage mammalian Promoter for TagRFP PCMV IE Host cells mammalian Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use red (orange) fluorescent labeling of cytokeratin-18

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Keratin: 600-1889
TagRFP: 1941-2654
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2807-2812 & 2836-2841
mRNA 3' ends: 2845 & 2857
f1 single-strand DNA origin: 2904-3359
Bacterial promoter for expression of Kan^r gene
-35 region: 3421-3426
-10 region: 3444-3449
Transcription start point: 3456
SV40 origin of replication: 3700-3835
SV40 early promoter
Enhancer (72-bp tandem repeats): 3533-3604 & 3605-3676
21-bp repeats: 3680-3700, 3701-3721 & 3723-3743
Early promoter element: 3756-3762
Major transcription start points: 3752, 3790, 3796 & 3801
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3884-3886
Stop codon: 4676-4678
G->A mutation to remove Pst I site: 4066
C->A (Arg to Ser) mutation to remove BssH II site: 4412
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4914-4919 & 4927-4932
pUC plasmid replication origin: 5263-5906