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RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs/TagRFP

pTagRFP-profilin

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pTagRFP-profilin vector

cat.# FP371

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pTagRFP-profilin	FP371	20 μg	€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector type	mammalian expression vector
Reporter	TagRFP
Reporter codon usage	mammalian
Promoter for TagRFP	P_{CMV IE}
Host cells	mammalian
Selection	prokaryotic - kanamycin eukaryotic - neomycin (G418)
Replication	prokaryotic - pUC ori eukaryotic - SV40 ori
Use	red (orange) fluorescent labeling of profilin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagRFP
Start codon (ATG): 613-615
Last amino acid in TagRFP: 1321-1323
Profilin: 1354-1776
Stop codon: 1774-1776
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1937-1942 & 1966-1971
mRNA 3' ends: 1975 & 1987
f1 single-strand DNA origin: 2034-2489
Bacterial promoter for expression of Kan^r gene
-35 region: 2551-2556
-10 region: 2574-2579
Transcription start point: 2586
SV40 origin of replication: 2830-2965
SV40 early promoter
Enhancer (72-bp tandem repeats): 2663-2734 & 2735-2806
21-bp repeats: 2810-2830, 2831-2851 & 2853-2873
Early promoter element: 2886-2892
Major transcription start points: 2882, 2920, 2926 & 2931
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3014-3016
Stop codon: 3806-3808
G->A mutation to remove Pst I site: 3196
C->A (Arg to Ser) mutation to remove BssH II site: 3542
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4044-4049 & 4057-4062
pUC plasmid replication origin: 4393-5036

Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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