pTagRFP-vinculin vector

cat.# FP372

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagRFP-vinculin FP372 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagRFP Reporter codon usage mammalian Promoter for TagRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red (orange) fluorescent labeling of vinculin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagRFP
Start codon (ATG): 613-615
Last amino acid in TagRFP: 1321-1323
Vinculin: 1393-4593
Stop codon: 4591-4593
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 4746-4751 & 4775-4780
mRNA 3' ends: 4784 & 4796
f1 single-strand DNA origin: 4843-5298
Bacterial promoter for expression of Kan^r gene
-35 region: 5360-5365
-10 region: 5383-5388
Transcription start point: 5395
SV40 origin of replication: 5639-5774
SV40 early promoter
Enhancer (72-bp tandem repeats): 5472-5543 & 5544-5615
21-bp repeats: 5619-5639, 5640-5660 & 5662-5682
Early promoter element: 5695-5701
Major transcription start points: 5691, 5729, 5735 & 5740
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 5823-5825
Stop codon: 6615-6617
G->A mutation to remove Pst I site: 6005
C->A (Arg to Ser) mutation to remove BssH II site: 6351
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 6853-6858 & 6866-6871
pUC plasmid replication origin: 7202-7845