pTurboFP602-PRL vector

cat.# FP715

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboFP602-PRL FP715 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter TurboFP602 Reporter codon usage mammalian Promoter for TurboFP602 NO Host cells mammalian, prokaryotic Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use Monitoring transcription from different promoters and promoter/enhancer combinations

Location of features

MCS: 10-89
TurboFP602
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 802-804
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 958-963 & 987-992
mRNA 3' ends: 996 & 1008
f1 single-strand DNA origin: 1055-1510
Eukaryotic promoter for expression of Kan^r gene
-35 region: 1572-1577
-10 region: 1595-1600
Transcription start point: 1607
SV40 origin of replication: 1851-1986
SV40 early promoter
Enhancer (72-bp tandem repeats): 1684-1755 & 1756-1827
21-bp repeats: 1831-1851, 1852-1872 & 1874-1894
Early promoter element: 1907-1913
Major transcription start points: 1903, 1941, 1947 & 1952
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2035-2037
Stop codon: 2827-2829
G->A mutation to remove Pst I site: 2217
C->A (Arg to Ser) mutation to remove BssH II site: 2563
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3065-3070 & 3078-3083
pUC plasmid replication origin: 3414-4057