Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboFP650
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1393-1395
Last amino acid in mKate2: 1312-1314
MCS: 1315-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1535-1540 & 1564-1569
mRNA 3' ends: 1573 & 1585
f1 single-strand DNA origin: 1632-2087
Bacterial promoter for expression of Kan^r gene
-35 region: 2149-2154
-10 region: 2172-2177
Transcription start point: 2184
SV40 origin of replication: 2428-2563
SV40 early promoter
Enhancer (72-bp tandem repeats): 2261-2332 & 2333-2404
21-bp repeats: 2408-2428, 2429-2449 & 2451-2471
Early promoter element: 2484-2490
Major transcription start points: 2480, 2518, 2524 & 2529
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2612-2614
Stop codon: 3404-3406
G->A mutation to remove Pst I site: 2794
C->A (Arg to Ser) mutation to remove BssH II site: 3140
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3642-3647 & 3655-3660
pUC plasmid replication origin: 3991-4634